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Prepare the stacking gel as follows: in disposable plastic tubes, prepare the appropriate volume of solution, containing the desired concentration of acrylamide. Polymerization will begin as soon as the TEMEO has been added. Without delay, swirl the mixture rapidly and proceed to the next step.

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Steps in Gel Preparation Prepare the gel casting cassette (wash and clean glass, treat with hydrophobic reagent if necessary, and assemble). Prepare the gel Pour the gel into the gel casting cassette. Allow a minimum of 2 hours prior to using the gel.

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Polyacrylamide (PAM) is a versatile polymer widely used in water treatment, papermaking, oil extraction and other fields. According to its ionic properties, PAM is divided into three main types: cationic (Cationic PAM, CPAM), anionic (Anionic PAM, APAM) and nonionic (Nonionic PAM, NPAM).

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Cationic polyacrylamide, is a white powder water -soluble polymer which is copolymerized by cationic monomer and acrylamide. It has the function of decolorizing, adsorbing, turbid eliminating and bonding. It generate a good flocculation and can decrease the resistance between liquids.

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PAM (anionic Polyacrylamide) for Runoff and Soil Erosion Control PAM, or anionic Polyacrylamide, is a very long chain, high molecular weight organic polymer produced from natural gas, with characteristics which make it useful as a soil amendment to control

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Anionic polyacrylamide since the structural unit contains an amide group, Anionic polyacrylamide is easy to form hydrogen bonds, so Anionic polyacrylamide has good water solubility. Anionic polyacrylamide is easy to obtain a variety of modified products with branched or network structure through grafting or crosslinking.

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Additionally, polyacrylamide can be employed in various stages of water treatment, including wastewater treatment and drinking water purification. Its ability to bind with pollutants ensures that contaminants are effectively removed, resulting in higher quality water

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